This hands-on course will focus on the training in genetic manipulation of zebrafish, including techniques for transgenesis and mutagenesis. The participants will be offered theoretical and practical sessions on Tol2-mediated transgenesis and Crispr-Cas9 mediated genome editing. Microinjection into zebrafish embryos will be covered, as well as previous preparation of RNA or DNA to inject and subsequent screening to identify positive founder fish.
- Teacher: Jose Bessa
- Teacher: Joana Pinheiro Marques
The aim of this workshop is to provide hands-on training in genome editing in mammalian cells using the powerful CRISPR/Cas9 system. It will cover topics from sgRNA design, molecular cloning and transfection, through off-target assessment, validation and different applications.
- Teacher: Joana Caldeira
- Teacher: Catarina Gomes