This hands-on course will focus on the training in genetic manipulation of zebrafish, including techniques for transgenesis and mutagenesis. The participants will be offered theoretical and practical sessions on Tol2-mediated transgenesis and Crispr-Cas9 mediated genome editing. Microinjection into zebrafish embryos will be covered, as well as previous preparation of RNA or DNA to inject and subsequent screening to identify positive founder fish.

The aim of this workshop is to provide hands-on training in genome editing in mammalian cells using the powerful CRISPR/Cas9 system. It will cover topics from sgRNA design, molecular cloning and transfection, through off-target assessment, validation and different applications.