Atomic Force Microscopy (AFM) has revealed as a powerful tool to study human pathology, in fields ranging from cancer, cardiovascular and blood diseases, since it is suitable to perform studies on different molecules, cell/ tissue types at physiological conditions. The main principle is based in the interatomic forces stablished between a probe tip and the sample causing the cantilever to deflect as the samples’ surface topography changes. A laser light reflected from the back of the cantilever measures the deflection of the cantilever. In this lab session the participants will be introduced to the AFM/IFM techniques, having the opportunity to learn about the determination of morphometric parameters to the characterization of biomechanical properties of cells. As well, students will learn how to use different software for the analysis of the data arising from these studies. Applets software will be used to analyze the Thermal tune data to obtain the force constant of the cantilevers and to analyze AFM force distance curves, to calculate the mechanical properties of the samples.
With the advent of digital era, nowadays images are multidimensional numerical data that can be processed and analyzed quantitatively to extract more information. ImageJ/Fiji open source software (imagej.net/) is a powerful tool available to process and analyze digital images. Moreover, it also allow the automation of image analysis with custom macros and plugins. With this course we intend to give a general introduction to ImageJ/Fiji, show how to use it to extract quantitative data and how to write simple macros. This course will be focused in the analysis of light microscopy images, but is targeted to all people engaged in research, that wants to start using ImageJ/Fiji for image analysis in general.