A comprehensive and integrated characterization of molecular interactions is key to the understanding of life processes at a molecular level. Due to their complexity the study of binding events requires orthogonal approaches, i.e. the gathering of data from multiple methods in order to build a complete picture of their function.

Hence this practical course aims at a multidisplinary approach to the study of molecular interactions from and in biological systems, integrating biochemical/biophysical methodologies with live microscopy techniques.

In this 3rd Edition the course will cover a range of methods used in the kinetics and thermodynamics characterization of binding events, such as:

• Isothermal Titration Calorimetry (ITC)
• Surface Plasmon Resonance (SPR)
• Microscale Thermophoresis (MST)
• Surface Acoustic Wave (SAW)
• Electro-Switchable Biosurfaces (switchSENSE)
• Biolayer Interferometry

The bridge to in vivo will be crossed with the introduction of advanced light microscopy techniques, e.g.:

• Fluorescence Resonance Energy Transfer (FRET)
• Fluorescence Lifetime Imaging Microscopy (FLIM)

to study the dynamic interactions of proteins within the living cell.