The Course on High Content Screening (HCS) and Image Analysis for Biosciences is a comprehensive and intensive course that aims to provide the basis of high-throughput screening (HTS) with emphasis on image-based screening. It is designed to graduate students, early career investigators and everyone willing to enter in the multiplex era.The course includes talks given by specialists in the field and hands-on sessions with HTS and HCS instruments and open-source software for image and data analysis.